Please contact OriDB if you think this paper has been mis-annotated.
| Replication profile of Saccharomyces cerevisiae chromosome VI. | ||
| K L Friedman, B J Brewer, W L Fangman | ||
| Genes Cells (1997), 2(11):667-78 | ||
| BACKGROUND: An understanding of the replication programme at the genome level will require the identification and characterization of origins of replication through large, contiguous regions of DNA. As a step toward this goal, origin efficiencies and replication times were determined for 10 ARSs spanning most of the 270 kilobase (kb) chromosome VI of Saccharomyces cerevisiae. RESULTS: Chromosome VI shows a wide variation in the percentage of cell cycles in which different replication origins are utilized. Most of the origins are activated in only a fraction of cells, suggesting that the pattern of origin usage on chromosome VI varies greatly within the cell population. The replication times of fragments containing chromosome VI origins show a temporal pattern that has been recognized on other chromosomes--the telomeres replicate late in S phase, while the central region of the chromosome replicates early. CONCLUSIONS: As demonstrated here for chromosome VI, analysis of the direction of replication fork movement along a chromosomeand determination of replication time by measuring a period of hemimethylation may provide an efficient means of surveying origin activity over large regions of the genome. | ||
OriDB annotation of this paper: |
||
Origin site(s) analysed by ARS assay: |
None curated. | |
Origin site(s) analysed by 2D gel analysis: |
ARS601, ARS at , ARS603, ARS603.5, ARS604, ARS605, ARS606, ARS607, ARS608, ARS609. | |
Predicted ACS element: |
None curated. | |
Experimentally confirmed ACS element: |
None curated. | |
Activation ('firing') time determined: |
ARS601, ARS at , ARS603, ARS603.5, ARS604, ARS605, ARS606, ARS607, ARS608, ARS609. | |
Activation ('firing') efficiency determined: |
ARS601, ARS at , ARS603, ARS603.5, ARS604, ARS605, ARS606, ARS607, ARS608, ARS609. | |
Analysed by microarray: |
None curated. | |
Other analysis: |
None curated. | |
|
||
S. cerevisiae OriDB - v1.3.0.
Designed and maintained by Dr Conrad A. Nieduszynski & Dr Anne D. Donaldson
