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| Time of replication of ARS elements along yeast chromosome III. | ||
| A E Reynolds, R M McCarroll, C S Newlon, W L Fangman | ||
| Mol Cell Biol (1989), 9(10):4488-94 | ||
| The replication of putative replication origins (ARS elements) was examined for 200 kilobases of chromosome III of Saccharomyces cerevisiae. By using synchronous cultures and transfers from dense to light isotope medium, the temporal pattern of mitotic DNA replication of eight fragments that contain ARSs was determined. ARS elements near the telomeres replicated late in S phase, while internal ARS elements replicated in the first half of S phase. The results suggest that some ARS elements in the chromosome may be inactive as replication origins. The actively expressed mating type locus, MAT, replicated early in S phase, while the silent cassettes, HML and HMR, replicated late. Unexpectedly, chromosome III sequences were found to replicate late in G1 at the arrest induced by the temperature-sensitive cdc7 allele. | ||
OriDB annotation of this paper: |
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Origin site(s) analysed by ARS assay: |
None curated. | |
Origin site(s) analysed by 2D gel analysis: |
None curated. | |
Predicted ACS element: |
None curated. | |
Experimentally confirmed ACS element: |
None curated. | |
Activation ('firing') time determined: |
ARS305, ARS307, ARS309. | |
Activation ('firing') efficiency determined: |
None curated. | |
Analysed by microarray: |
None curated. | |
Other analysis: |
None curated. | |
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