Please contact OriDB if you think this paper has been mis-annotated.
| An ARS element inhibits DNA replication through a SIR2-dependent mechanism. | ||
| Amber Crampton, FuJung Chang, Donald L Pappas, Ryan L Frisch, Michael Weinreich | ||
| Mol Cell (2008), 30(2):156-66 | ||
| During G1 phase, a prereplicative complex (pre-RC) that determines where DNA synthesis initiates forms at origins. The Sir2p histone deacetylase inhibits pre-RC assembly at a subset of origins, suggesting that Sir2p inhibits DNA replication through a unique aspect of origin structure. Here, we identified five SIR2-sensitive origins on chromosomes III and VI. Linker scan analysis of two origins indicated that they share a common organization, including an inhibitory sequence positioned 3' to the sites of origin recognition complex (ORC) binding and pre-RC assembly. This inhibitory sequence (I(S)) required SIR2 for its activity, suggesting that SIR2 inhibits origins through this sequence. Furthermore, I(S) elements occurred within positioned nucleosomes, and Abf1p-mediated exclusion of nucleosomes from the origin abrogated the inhibition. These data suggest that Sir2p and I(S) elements inhibit origin activity by promoting an unfavorable chromatin structure for pre-RC assembly. | ||
OriDB annotation of this paper: |
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Origin site(s) analysed by ARS assay: |
ARS305, ARS315. | |
Origin site(s) analysed by 2D gel analysis: |
None curated. | |
Predicted ACS element: |
None curated. | |
Experimentally confirmed ACS element: |
ARS305, ARS315. | |
Activation ('firing') time determined: |
None curated. | |
Activation ('firing') efficiency determined: |
None curated. | |
Analysed by microarray: |
None curated. | |
Other analysis: |
None curated. | |
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