Please contact OriDB if you think this paper has been mis-annotated.
| Completion of replication map of Saccharomyces cerevisiae chromosome III. | ||
| A Poloumienko, A Dershowitz, J De, C S Newlon | ||
| Mol Biol Cell (2001), 12(11):3317-27 | ||
| In Saccharomyces cerevisiae chromosomal DNA replication initiates at intervals of approximately 40 kb and depends upon the activity of autonomously replicating sequence (ARS) elements. The identification of ARS elements and analysis of their function as chromosomal replication origins requires the use of functional assays because they are not sufficiently similar to identify by DNA sequence analysis. To complete the systematic identification of ARS elements on S. cerevisiae chromosome III, overlapping clones covering 140 kb of the right arm were tested for their ability to promote extrachromosomal maintenance of plasmids. Examination of chromosomal replication intermediates of each of the seven ARS elements identified revealed that their efficiencies of use as chromosomal replication origins varied widely, with four ARS elements active in |
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OriDB annotation of this paper: |
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Origin site(s) analysed by ARS assay: |
ARS313, ARS314, ARS315, ARS316, ARS319. | |
Origin site(s) analysed by 2D gel analysis: |
ARS313, ARS314, ARS315, ARS316, ARS317, ARS318. | |
Predicted ACS element: |
None curated. | |
Experimentally confirmed ACS element: |
None curated. | |
Activation ('firing') time determined: |
None curated. | |
Activation ('firing') efficiency determined: |
None curated. | |
Analysed by microarray: |
None curated. | |
Other analysis: |
None curated. | |
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